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Giving Evolutionary Biologists the Finger! KRAB Zinc Finger Domains and Violations of the Law of Large Numbers (
The evolutionary explanation of these patterns shows a severe lack of critical thinking and appreciation science from first principles of physics, chemistry, and mathematics, and even basic common sense.
This is an amino acid sequence in the ZNF136 protein
TGEKLYDCKECGKTFFSLKRIRRHIITHThis short sequence is called a Zinc Finger which in 3D looks like this:
Wiki gives a description of the function of Zinc Fingers in proteins that have them: https://en.wikipedia.org/wiki/Zinc_finger
A Zinc Finger requires two "C" amino acids and two "H" amino acids to be placed in the right positions. It requires a few other things, but these are the necessary features of a Zinc Finger.
This is the amino acid sequence of human ZNF136: https://www.uniprot.org/uniprot/P52737.fasta
There are 13 zinc fingers in the ZNF136 protein and these are their sequences:
TGEKLYDCKECGKTFFSLKRIRRHIITHTo visualize the critical amino acids, see the protein sequence here with highlights on "C" and "H" amino acids. Note one of the lines is not exactly like the other lines in that it is missing a "C", and is thus considered a degenerate zinc finger. So there is 1 degenerate zinc finger and 13 functional ones.
Hopefully it is apparent that the regular appearance of "C" and "H" is a violation of the law of large numbers, hence this pattern is not due to random mutation.
The Darwinist explanation of this pattern is segment duplication and natural selection. But Darwinists show rather flimsy critical thinking skills in their explanation of this repeating pattern.
To understand why, let the reader ponder the alignment I made of the Zinc Fingers in the ZNF136 protein using MEGA 6.0/MUSCLE software:
This is the distance matrix generated by MEGA 6.0 which measures the number of nucleotide and percent differences between the zinc fingers.
All of the above results are reproducible, so I leave it to interested parties wanting to confirm the results to do so.
So the duplications are not exact. Ok, so the Joe Darwinsit will say, "well one zinc finger was duplicated and then later changed their 28 amino acid sequences." The problem with that is if there is random mutation, why are the Zinc Finger's preserved and not erased? To preserve the "C" and "H" positions they need to be at least under selection. But then one needs to invoke a just so story that a newly minted zinc finger has function.
Furthermore, why is the supposed copied segment repeat exactly 28 amino acids, which would require 84 nucleotides? Not only must the 84 nucleotides be copied, they have to be inserted in the right place, otherwise disaster happens.
So what do zinc fingers do? Well, among other things they bind to DNAs regions including DNAs such as ERVs!!!!
Here is a conceptual depiction of a KRAB-ZNF Protein with a mere 4 zinc fingers binding to DNA. Look for the bubble with the word "KRAB" and "KZNF" (for Krab Zinc Finger):
Note the four "ZN" fingers attaching to the DNAs! So this is a hypothetical KRAB-ZNF protein that has 4 fingers to grab a SPECIFIC pattern of DNA. The KRAB-ZNF protein is part of an incredibly complex machine that does chromatin modification. This KRAB-ZNF is like a read/write head acting on Chromatin. Chromatin itself is an amazing mind-boggling design akin to computer ROM and RAM in one!
Random insertion mutations and point mutations would disrupt the binding of an already operational zinc finger. Adding and preserving new zinc fingers through natural selection would entail having fortuitous DNA targets that make the new zinc finger functional.
This is like making random changes to a lock (a complex zinc finger protein) and expecting a random key to open it!
Finally why are the Zinc Fingers slightly different in sequence? It turns out there is a Zinc Finger code!
To target a section of DNA, the zinc finger must be tuned to target it. Think of the zinc finger like lock and DNA as the key that fits into the lock! In fact, for both the study of biology and medical applications, humans have a desire to make their own zinc fingers -- like lock smiths.
There is a website that helps researches construct the right amino acid sequence to make a zinc finger with a particular DNA target:
In sum, there is a violation of the law of large numbers in KRAB-zinc finger proteins like ZNF136 which is not explained by random mutation, random segment duplication, nor natural selection. Some other mechanism for the emergence of such proteins is indicated. Given the importance of such zinc finger proteins in the control of ERVs which are important in the stem cell pluripotency regulatory circuits, this is even more of a miracle.
[I'm invoking ARN Rule 9 and am banning people from this thread who are on my block list from participating. If they want to object to anything I say, they are welcome to start their own thread and run it according to their rules and say whatever is on their mind. They can even ban me from their threads!
A list of people on my block list is here: https://www.reddit.com/CreationEvolution/comments/alkjl6/policy_on_who_i_ignore_and_an_offer_to_sincere/ejkv9id/ ]